Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5293751..5294248 Replicon chromosome
Accession NZ_AP027170
Organism Escherichia coli strain 20.1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG87_RS26950 Protein ID WP_001173343.1
Coordinates 5294075..5294248 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG87_RS26945 Protein ID WP_000398619.1
Coordinates 5293751..5294047 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG87_RS26925 (5290482) 5290482..5291201 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG87_RS26930 (5291198) 5291198..5292829 - 1632 WP_001216487.1 sensor histidine kinase -
QMG87_RS26935 (5293010) 5293010..5293240 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG87_RS26940 (5293252) 5293252..5293524 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG87_RS26945 (5293751) 5293751..5294047 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG87_RS26950 (5294075) 5294075..5294248 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG87_RS26955 (5294367) 5294367..5295884 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG87_RS26960 (5296121) 5296121..5297578 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43231 WP_001173343.1 NZ_AP027170:5294075-5294248 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43231 NZ_AP027170:5294075-5294248 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43231 WP_000398619.1 NZ_AP027170:5293751-5294047 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43231 NZ_AP027170:5293751-5294047 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References