Detailed information of TA system TA043228

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3921540..3922194
• Replicon: chromosome
• Accession: NZ_AP027170
• Organism: Escherichia coli strain 20.1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: QMG87_RS20375
• Protein ID: WP_000244777.1
• Coordinates: 3921540..3921947 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: QMG87_RS20380
• Protein ID: WP_000354043.1
• Coordinates: 3921928..3922194 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG87_RS20355 (3917497)	3917497..3919230	-	1734	WP_000813220.1	single-stranded-DNA-specific exonuclease RecJ	-
QMG87_RS20360 (3919236)	3919236..3919946	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
QMG87_RS20365 (3919971)	3919971..3920867	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
QMG87_RS20370 (3920979)	3920979..3921500	+	522	WP_001055874.1	flavodoxin FldB	-
QMG87_RS20375 (3921540)	3921540..3921947	-	408	WP_000244777.1	protein YgfX	Toxin
QMG87_RS20380 (3921928)	3921928..3922194	-	267	WP_000354043.1	FAD assembly factor SdhE	Antitoxin
QMG87_RS20385 (3922437)	3922437..3923417	+	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
QMG87_RS20390 (3923613)	3923613..3924272	-	660	WP_000250274.1	hemolysin III family protein	-
QMG87_RS20395 (3924436)	3924436..3924747	-	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
QMG87_RS20400 (3924792)	3924792..3926225	+	1434	WP_001341859.1	6-phospho-beta-glucosidase BglA	-
QMG87_RS20405 (3926282)	3926282..3927025	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T43228 WP_000244777.1 NZ_AP027170:c3921947-3921540 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T43228 NZ_AP027170:c3921947-3921540 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10563.16 Da
• Isoelectric Point: 5.1599

>AT43228 WP_000354043.1 NZ_AP027170:c3922194-3921928 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT43228 NZ_AP027170:c3922194-3921928 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7