Detailed information of TA system TA043225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3418513..3419138
• Replicon: chromosome
• Accession: NZ_AP027170
• Organism: Escherichia coli strain 20.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG87_RS17860
• Protein ID: WP_000911330.1
• Coordinates: 3418513..3418911 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG87_RS17865
• Protein ID: WP_000450524.1
• Coordinates: 3418911..3419138 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG87_RS17840 (3414391)	3414391..3414591	+	201	WP_000383836.1	YpfN family protein	-
QMG87_RS17845 (3414701)	3414701..3415399	-	699	WP_000679812.1	esterase	-
QMG87_RS17850 (3415473)	3415473..3417488	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG87_RS17855 (3417503)	3417503..3418366	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG87_RS17860 (3418513)	3418513..3418911	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG87_RS17865 (3418911)	3418911..3419138	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG87_RS17870 (3419292)	3419292..3420005	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG87_RS17875 (3420218)	3420218..3421252	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG87_RS17880 (3421269)	3421269..3422147	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG87_RS17885 (3422293)	3422293..3422865	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG87_RS17890 (3422865)	3422865..3423335	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43225 WP_000911330.1 NZ_AP027170:c3418911-3418513 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43225 NZ_AP027170:c3418911-3418513 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43225 WP_000450524.1 NZ_AP027170:c3419138-3418911 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43225 NZ_AP027170:c3419138-3418911 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33