Detailed information of TA system TA043201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 484096..484933
• Replicon: chromosome
• Accession: NZ_AP027170
• Organism: Escherichia coli strain 20.1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QMG87_RS02280
• Protein ID: WP_000227784.1
• Coordinates: 484096..484638 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QMG87_RS02285
• Protein ID: WP_001297137.1
• Coordinates: 484622..484933 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG87_RS02260 (479635)	479635..480546	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QMG87_RS02265 (480714)	480714..481205	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QMG87_RS02270 (481333)	481333..482697	-	1365	WP_001000977.1	MFS transporter	-
QMG87_RS02275 (483105)	483105..484040	+	936	WP_001375746.1	tetratricopeptide repeat protein	-
QMG87_RS02280 (484096)	484096..484638	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QMG87_RS02285 (484622)	484622..484933	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QMG87_RS02290 (485118)	485118..486008	-	891	WP_000971336.1	heme o synthase	-
QMG87_RS02295 (486020)	486020..486349	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QMG87_RS02300 (486349)	486349..486963	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QMG87_RS02305 (486953)	486953..488944	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QMG87_RS02310 (488966)	488966..489913	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43201 WP_000227784.1 NZ_AP027170:c484638-484096 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43201 NZ_AP027170:c484638-484096 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43201 WP_001297137.1 NZ_AP027170:c484933-484622 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43201 NZ_AP027170:c484933-484622 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3