Detailed information of TA system TA043184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3329744..3330369
• Replicon: chromosome
• Accession: NZ_AP027165
• Organism: Escherichia coli strain H19

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG77_RS17265
• Protein ID: WP_000911330.1
• Coordinates: 3329744..3330142 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG77_RS17270
• Protein ID: WP_000450524.1
• Coordinates: 3330142..3330369 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG77_RS17245 (3325622)	3325622..3325822	+	201	WP_000383836.1	YpfN family protein	-
QMG77_RS17250 (3325932)	3325932..3326630	-	699	WP_000679812.1	esterase	-
QMG77_RS17255 (3326704)	3326704..3328719	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG77_RS17260 (3328734)	3328734..3329597	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG77_RS17265 (3329744)	3329744..3330142	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG77_RS17270 (3330142)	3330142..3330369	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG77_RS17275 (3330523)	3330523..3331236	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG77_RS17280 (3331449)	3331449..3332483	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG77_RS17285 (3332500)	3332500..3333378	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG77_RS17290 (3333524)	3333524..3334096	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG77_RS17295 (3334096)	3334096..3334566	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43184 WP_000911330.1 NZ_AP027165:c3330142-3329744 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43184 NZ_AP027165:c3330142-3329744 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43184 WP_000450524.1 NZ_AP027165:c3330369-3330142 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43184 NZ_AP027165:c3330369-3330142 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33