Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3979678..3980332 Replicon chromosome
Accession NZ_AP027162
Organism Escherichia coli strain EH031

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QMG85_RS20655 Protein ID WP_000244777.1
Coordinates 3979678..3980085 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QMG85_RS20660 Protein ID WP_000354046.1
Coordinates 3980066..3980332 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG85_RS20635 (3975635) 3975635..3977368 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
QMG85_RS20640 (3977374) 3977374..3978084 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QMG85_RS20645 (3978109) 3978109..3979005 - 897 WP_024946574.1 site-specific tyrosine recombinase XerD -
QMG85_RS20650 (3979117) 3979117..3979638 + 522 WP_001055874.1 flavodoxin FldB -
QMG85_RS20655 (3979678) 3979678..3980085 - 408 WP_000244777.1 protein YgfX Toxin
QMG85_RS20660 (3980066) 3980066..3980332 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QMG85_RS20665 (3980575) 3980575..3981555 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
QMG85_RS20670 (3981751) 3981751..3982410 - 660 WP_000250274.1 hemolysin III family protein -
QMG85_RS20675 (3982574) 3982574..3982885 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QMG85_RS20680 (3982930) 3982930..3984363 + 1434 WP_141242804.1 6-phospho-beta-glucosidase BglA -
QMG85_RS20685 (3984420) 3984420..3985163 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T43151 WP_000244777.1 NZ_AP027162:c3980085-3979678 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43151 NZ_AP027162:c3980085-3979678 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43151 WP_000354046.1 NZ_AP027162:c3980332-3980066 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43151 NZ_AP027162:c3980332-3980066 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References