Detailed information of TA system TA043148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3480734..3481359
• Replicon: chromosome
• Accession: NZ_AP027162
• Organism: Escherichia coli strain EH031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG85_RS18200
• Protein ID: WP_000911330.1
• Coordinates: 3480734..3481132 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG85_RS18205
• Protein ID: WP_000450524.1
• Coordinates: 3481132..3481359 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG85_RS18180 (3476612)	3476612..3476812	+	201	WP_000383836.1	YpfN family protein	-
QMG85_RS18185 (3476922)	3476922..3477620	-	699	WP_000679812.1	esterase	-
QMG85_RS18190 (3477694)	3477694..3479709	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG85_RS18195 (3479724)	3479724..3480587	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG85_RS18200 (3480734)	3480734..3481132	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG85_RS18205 (3481132)	3481132..3481359	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG85_RS18210 (3481513)	3481513..3482226	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG85_RS18215 (3482439)	3482439..3483473	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG85_RS18220 (3483490)	3483490..3484368	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG85_RS18225 (3484514)	3484514..3485086	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG85_RS18230 (3485086)	3485086..3485556	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43148 WP_000911330.1 NZ_AP027162:c3481132-3480734 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43148 NZ_AP027162:c3481132-3480734 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43148 WP_000450524.1 NZ_AP027162:c3481359-3481132 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43148 NZ_AP027162:c3481359-3481132 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33