Detailed information of TA system TA043124

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 375255..376089
• Replicon: chromosome
• Accession: NZ_AP027162
• Organism: Escherichia coli strain EH031

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: QMG85_RS01725
• Protein ID: WP_024224470.1
• Coordinates: 375712..376089 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: QMG85_RS01720
• Protein ID: WP_024224469.1
• Coordinates: 375255..375623 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG85_RS01685 (371441)	371441..372118	+	678	WP_044864962.1	hypothetical protein	-
QMG85_RS01690 (372134)	372134..372544	+	411	WP_000846712.1	hypothetical protein	-
QMG85_RS01695 (372765)	372765..373583	+	819	WP_044808189.1	DUF932 domain-containing protein	-
QMG85_RS01700 (373583)	373583..373756	+	174	WP_001183321.1	hypothetical protein	-
QMG85_RS01705 (373922)	373922..374395	+	474	WP_024224468.1	antirestriction protein	-
QMG85_RS01710 (374410)	374410..374886	+	477	WP_044864720.1	RadC family protein	-
QMG85_RS01715 (374955)	374955..375176	+	222	WP_000692312.1	DUF987 domain-containing protein	-
QMG85_RS01720 (375255)	375255..375623	+	369	WP_024224469.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QMG85_RS01725 (375712)	375712..376089	+	378	WP_024224470.1	TA system toxin CbtA family protein	Toxin
QMG85_RS01730 (376086)	376086..376574	+	489	WP_024224471.1	DUF5983 family protein	-
QMG85_RS01735 (376586)	376586..376783	+	198	WP_024224472.1	DUF957 domain-containing protein	-
QMG85_RS01740 (376868)	376868..377716	+	849	WP_024224473.1	DUF4942 domain-containing protein	-
QMG85_RS01745 (377809)	377809..379128	-	1320	WP_000144688.1	site-specific integrase	-
QMG85_RS01750 (379944)	379944..380102	+	159	WP_000788777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13976.03 Da
• Isoelectric Point: 7.3580

>T43124 WP_024224470.1 NZ_AP027162:375712-376089 [Escherichia coli]
MKTLPVLPGLAASSHPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAEAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T43124 NZ_AP027162:375712-376089 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCACCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGAAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13625.47 Da
• Isoelectric Point: 6.4768

>AT43124 WP_024224469.1 NZ_AP027162:375255-375623 [Escherichia coli]
VSDILPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT43124 NZ_AP027162:375255-375623 [Escherichia coli]
GTGTCAGACATACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA