Detailed information of TA system TA043121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9823..10466
• Replicon: plasmid pO26_2313_2
• Accession: NZ_AP027161
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: QMG94_RS29740
• Protein ID: WP_001044768.1
• Coordinates: 10050..10466 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: QMG94_RS29735
• Protein ID: WP_001261287.1
• Coordinates: 9823..10053 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS29705 (T2313_p2_04)	5688..7127	+	1440	WP_001213544.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
QMG94_RS29710	7194..7337	+	144	Protein_4	transcriptional regulator	-
QMG94_RS29715 (T2313_p2_05)	7341..7532	+	192	Protein_5	lipid II-degrading bacteriocin	-
QMG94_RS29720 (T2313_p2_06)	7540..7893	-	354	WP_000864810.1	colicin M immunity protein	-
QMG94_RS29725 (T2313_p2_07)	8066..8848	-	783	WP_001164205.1	site-specific integrase	-
QMG94_RS29730 (T2313_p2_08)	8850..9263	-	414	WP_000465041.1	hypothetical protein	-
QMG94_RS29735 (T2313_p2_09)	9823..10053	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
QMG94_RS29740 (T2313_p2_10)	10050..10466	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG94_RS29745 (T2313_p2_11)	10628..11316	-	689	Protein_11	hypothetical protein	-
QMG94_RS29750 (T2313_p2_12)	11931..12791	-	861	WP_000704534.1	alpha/beta hydrolase	-
QMG94_RS29755 (T2313_p2_13)	12919..13275	+	357	Protein_13	tyrosine-type recombinase/integrase	-
QMG94_RS29760 (T2313_p2_14)	13359..14033	+	675	WP_001341423.1	IS66-like element accessory protein TnpA	-
QMG94_RS29765 (T2313_p2_15)	14030..14377	+	348	WP_000631725.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP	1..88893	88893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T43121 WP_001044768.1 NZ_AP027161:10050-10466 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T43121 NZ_AP027161:10050-10466 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT43121 WP_001261287.1 NZ_AP027161:9823-10053 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT43121 NZ_AP027161:9823-10053 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4