Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5328902..5329399 Replicon chromosome
Accession NZ_AP027159
Organism Escherichia coli strain 2313

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG94_RS27135 Protein ID WP_001173343.1
Coordinates 5329226..5329399 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG94_RS27130 Protein ID WP_000398619.1
Coordinates 5328902..5329198 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG94_RS27110 (5325633) 5325633..5326352 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG94_RS27115 (5326349) 5326349..5327980 - 1632 WP_001216487.1 sensor histidine kinase -
QMG94_RS27120 (5328161) 5328161..5328391 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG94_RS27125 (5328403) 5328403..5328675 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG94_RS27130 (5328902) 5328902..5329198 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG94_RS27135 (5329226) 5329226..5329399 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG94_RS27140 (5329518) 5329518..5331035 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG94_RS27145 (5331272) 5331272..5332729 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espG / escE / cesAB / escK / escL / escR / escS / escT / escU / etgA / cesD / escC / sepD / escJ / escI / sepZ/espZ / cesL / escV / escN / escO / sepQ/escQ / espH / cesF / map / tir / cesT / eae / escD / sepL / espA / espD / espB / cesD2 / escF / escG / espF / efa1 / nleE / nleB1 / espL2 5328902..5397370 68468


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43115 WP_001173343.1 NZ_AP027159:5329226-5329399 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43115 NZ_AP027159:5329226-5329399 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43115 WP_000398619.1 NZ_AP027159:5328902-5329198 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43115 NZ_AP027159:5328902-5329198 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References