Detailed information of TA system TA043109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3438551..3439176
• Replicon: chromosome
• Accession: NZ_AP027159
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG94_RS17950
• Protein ID: WP_000911330.1
• Coordinates: 3438551..3438949 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG94_RS17955
• Protein ID: WP_000450524.1
• Coordinates: 3438949..3439176 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS17930 (3434429)	3434429..3434629	+	201	WP_000383836.1	YpfN family protein	-
QMG94_RS17935 (3434739)	3434739..3435437	-	699	WP_000679812.1	esterase	-
QMG94_RS17940 (3435511)	3435511..3437526	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG94_RS17945 (3437541)	3437541..3438404	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG94_RS17950 (3438551)	3438551..3438949	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG94_RS17955 (3438949)	3438949..3439176	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG94_RS17960 (3439330)	3439330..3440043	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG94_RS17965 (3440256)	3440256..3441290	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG94_RS17970 (3441307)	3441307..3442185	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG94_RS17975 (3442331)	3442331..3442903	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG94_RS17980 (3442903)	3442903..3443373	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43109 WP_000911330.1 NZ_AP027159:c3438949-3438551 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43109 NZ_AP027159:c3438949-3438551 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43109 WP_000450524.1 NZ_AP027159:c3439176-3438949 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43109 NZ_AP027159:c3439176-3438949 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33