Detailed information of TA system TA043107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1943494..1944132
• Replicon: chromosome
• Accession: NZ_AP027159
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG94_RS09870
• Protein ID: WP_000813794.1
• Coordinates: 1943956..1944132 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG94_RS09865
• Protein ID: WP_001270286.1
• Coordinates: 1943494..1943910 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS09845 (1938647)	1938647..1939588	-	942	WP_001251332.1	ABC transporter permease	-
QMG94_RS09850 (1939589)	1939589..1940602	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG94_RS09855 (1940620)	1940620..1941765	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG94_RS09860 (1942010)	1942010..1943415	-	1406	Protein_1929	PLP-dependent aminotransferase family protein	-
QMG94_RS09865 (1943494)	1943494..1943910	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG94_RS09870 (1943956)	1943956..1944132	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG94_RS09875 (1944354)	1944354..1944584	+	231	WP_000494244.1	YncJ family protein	-
QMG94_RS09880 (1944676)	1944676..1946637	-	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG94_RS09885 (1946710)	1946710..1947246	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG94_RS09890 (1947299)	1947299..1948513	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1948553..1949701	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43107 WP_000813794.1 NZ_AP027159:c1944132-1943956 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43107 NZ_AP027159:c1944132-1943956 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43107 WP_001270286.1 NZ_AP027159:c1943910-1943494 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43107 NZ_AP027159:c1943910-1943494 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB