Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 528811..529429 Replicon chromosome
Accession NZ_AP027159
Organism Escherichia coli strain 2313

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QMG94_RS02500 Protein ID WP_001291435.1
Coordinates 528811..529029 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QMG94_RS02505 Protein ID WP_000344800.1
Coordinates 529055..529429 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG94_RS02465 (524101) 524101..524673 + 573 WP_000779826.1 YbaY family lipoprotein -
QMG94_RS02470 (524704) 524704..525015 - 312 WP_000409911.1 MGMT family protein -
QMG94_RS02480 (525393) 525393..525746 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QMG94_RS02485 (525788) 525788..527338 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QMG94_RS02490 (527502) 527502..527972 - 471 WP_000136192.1 YlaC family protein -
QMG94_RS02495 (528088) 528088..528639 - 552 WP_000102557.1 maltose O-acetyltransferase -
QMG94_RS02500 (528811) 528811..529029 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QMG94_RS02505 (529055) 529055..529429 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QMG94_RS02510 (529975) 529975..533124 - 3150 WP_001132475.1 efflux RND transporter permease AcrB -
QMG94_RS02515 (533147) 533147..534340 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43087 WP_001291435.1 NZ_AP027159:c529029-528811 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43087 NZ_AP027159:c529029-528811 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43087 WP_000344800.1 NZ_AP027159:c529429-529055 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43087 NZ_AP027159:c529429-529055 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References