Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1941..2488 Replicon plasmid pJNE181771-2
Accession NZ_AP027158
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag QUD66_RS24560 Protein ID WP_001384452.1
Coordinates 2210..2488 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2W8GN83
Locus tag QUD66_RS24555 Protein ID WP_000079940.1
Coordinates 1941..2210 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS24550 (1) 1..1032 + 1032 WP_000907875.1 plasmid replication initiator RepA -
QUD66_RS24555 (1941) 1941..2210 + 270 WP_000079940.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
QUD66_RS24560 (2210) 2210..2488 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
QUD66_RS24565 (2688) 2688..2954 - 267 WP_000627828.1 hypothetical protein -
QUD66_RS24570 (2954) 2954..3448 - 495 WP_021561690.1 hypothetical protein -
QUD66_RS24575 (3504) 3504..4106 - 603 WP_000517686.1 ProQ/FINO family protein -
QUD66_RS24580 (4215) 4215..4382 + 168 Protein_6 colicin-like pore-forming protein -
QUD66_RS24585 (4413) 4413..4748 - 336 WP_001080730.1 colicin 1A immunity protein -
QUD66_RS24590 (4877) 4877..5224 + 348 WP_000142456.1 DUF6404 family protein -
QUD66_RS24595 (5242) 5242..5832 - 591 WP_000198612.1 hypothetical protein -
QUD66_RS24600 (5832) 5832..6089 - 258 WP_000343101.1 hypothetical protein -
QUD66_RS24605 (6659) 6659..7072 + 414 WP_000465043.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88936 88936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T43080 WP_001384452.1 NZ_AP027158:2210-2488 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T43080 NZ_AP027158:2210-2488 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAATTGGACGAACGGATTGGTTATTCAGCCAGCAGCTTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10274.60 Da        Isoelectric Point: 7.2057

>AT43080 WP_000079940.1 NZ_AP027158:1941-2210 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDMLSHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT43080 NZ_AP027158:1941-2210 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGTCGTCAGCAACTCGAACGCGGTGATATGCTTTCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A2W8GN83

References