Detailed information of TA system TA043079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 126410..126935
• Replicon: plasmid pJNE181771-1
• Accession: NZ_AP027157
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: QUD66_RS24505
• Protein ID: WP_001159871.1
• Coordinates: 126410..126715 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: QUD66_RS24510
• Protein ID: WP_000813639.1
• Coordinates: 126717..126935 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS24475 (121677)	121677..122947	+	1271	Protein_134	translesion error-prone DNA polymerase V subunit UmuC	-
QUD66_RS24480 (122952)	122952..123344	-	393	WP_000340836.1	plasmid partitioning/stability family protein	-
QUD66_RS24485 (123349)	123349..124320	-	972	WP_001103695.1	plasmid segregation protein ParM	-
QUD66_RS24490 (124549)	124549..125193	+	645	WP_000633912.1	division plane positioning ATPase MipZ	-
QUD66_RS24495 (125187)	125187..125462	+	276	WP_000239527.1	hypothetical protein	-
QUD66_RS24500 (125600)	125600..126409	-	810	WP_000016958.1	site-specific integrase	-
QUD66_RS24505 (126410)	126410..126715	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUD66_RS24510 (126717)	126717..126935	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUD66_RS24515 (127531)	127531..127791	+	261	WP_000343084.1	hypothetical protein	-
QUD66_RS24520 (127788)	127788..128378	+	591	WP_000194564.1	hypothetical protein	-
QUD66_RS24525 (128396)	128396..128743	-	348	WP_000142428.1	DUF6404 family protein	-
QUD66_RS24530 (128997)	128997..129206	+	210	WP_225303885.1	colicin E1 immunity protein	-
QUD66_RS24535 (129213)	129213..129533	-	321	WP_001365556.1	colicin-like pore-forming protein	-
QUD66_RS24540 (129643)	129643..130374	-	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
QUD66_RS24545 (130608)	130608..130825	-	218	Protein_148	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..131387	131387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T43079 WP_001159871.1 NZ_AP027157:c126715-126410 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43079 NZ_AP027157:c126715-126410 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT43079 WP_000813639.1 NZ_AP027157:c126935-126717 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43079 NZ_AP027157:c126935-126717 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5