Detailed information of TA system TA043075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 70588..71213
• Replicon: plasmid pJNE181771-1
• Accession: NZ_AP027157
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUD66_RS24125
• Protein ID: WP_000911320.1
• Coordinates: 70815..71213 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2A2C6V4
• Locus tag: QUD66_RS24120
• Protein ID: WP_000450527.1
• Coordinates: 70588..70815 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS24120 (70588)	70588..70815	+	228	WP_000450527.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUD66_RS24125 (70815)	70815..71213	+	399	WP_000911320.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUD66_RS24130 (71222)	71222..73492	-	2271	WP_244058874.1	type IV conjugative transfer system coupling protein TraD	-
QUD66_RS24135 (73549)	73549..74280	-	732	WP_000199918.1	hypothetical protein	-
QUD66_RS24140 (74456)	74456..75244	-	789	WP_033817126.1	hypothetical protein	-
QUD66_RS24145 (75412)	75412..75723	+	312	WP_000522220.1	heavy metal-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..131387	131387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T43075 WP_000911320.1 NZ_AP027157:70815-71213 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDATAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T43075 NZ_AP027157:70815-71213 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTACTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTAACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8542.54 Da
• Isoelectric Point: 4.4013

>AT43075 WP_000450527.1 NZ_AP027157:70588-70815 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSDDFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT43075 NZ_AP027157:70588-70815 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCTGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGACGATTTTATGGATAACAGGGAACAGCCCGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A2C6V4