Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 4779020..4779798 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name ypjF Uniprot ID A0A3U1V6G9
Locus tag QUD66_RS23215 Protein ID WP_033805147.1
Coordinates 4779424..4779798 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3U1V6I4
Locus tag QUD66_RS23210 Protein ID WP_033805146.1
Coordinates 4779020..4779385 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS23165 (4774317) 4774317..4775021 + 705 WP_033805137.1 WYL domain-containing protein -
QUD66_RS23170 (4775021) 4775021..4775590 + 570 WP_033805138.1 hypothetical protein -
QUD66_RS23175 (4775627) 4775627..4776079 + 453 WP_033805139.1 hypothetical protein -
QUD66_RS23180 (4776076) 4776076..4776525 + 450 WP_033805140.1 IrmA family protein -
QUD66_RS23185 (4776597) 4776597..4776827 + 231 WP_033805141.1 DUF905 domain-containing protein -
QUD66_RS23190 (4776929) 4776929..4777750 + 822 WP_047082063.1 DUF932 domain-containing protein -
QUD66_RS23195 (4777781) 4777781..4778221 + 441 WP_033805143.1 antirestriction protein -
QUD66_RS23200 (4778234) 4778234..4778776 + 543 WP_033805144.1 DNA repair protein RadC -
QUD66_RS23205 (4778773) 4778773..4778994 + 222 WP_033805145.1 DUF987 domain-containing protein -
QUD66_RS23210 (4779020) 4779020..4779385 + 366 WP_033805146.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
QUD66_RS23215 (4779424) 4779424..4779798 + 375 WP_033805147.1 TA system toxin CbtA family protein Toxin
QUD66_RS23220 (4780295) 4780295..4781559 + 1265 Protein_4525 tyrosine-type recombinase/integrase -
QUD66_RS23225 (4781761) 4781761..4783230 - 1470 WP_001022619.1 serine/threonine-protein kinase -
QUD66_RS23230 (4783227) 4783227..4783769 - 543 WP_001115385.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC 4772365..4810188 37823


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-113)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13843.88 Da        Isoelectric Point: 7.9537

>T43072 WP_033805147.1 NZ_AP027156:4779424-4779798 [Escherichia coli]
MQTISSHLTRAAQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFSDETTIQKHIDAGISLSDAVNFLVEKYELIRIDRDGC
SVMAQKSPFITSIDILRARKASGLMKRSSHKTVTRATAGQHQEL

Download         Length: 375 bp

>T43072 NZ_AP027156:4779424-4779798 [Escherichia coli]
ATGCAAACCATATCATCACACCTGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTGATGAAACCACCATCCAGAAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATATGAACTGATAAGAATTGACCGCGATGGTTGC
TCTGTCATGGCGCAGAAGTCACCGTTTATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACG
CAGCAGTCACAAAACTGTTACCAGGGCAACGGCAGGCCAACATCAGGAGCTATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13393.06 Da        Isoelectric Point: 5.0700

>AT43072 WP_033805146.1 NZ_AP027156:4779020-4779385 [Escherichia coli]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEADTLGSCGYVFIAIYPEQTEPQ

Download         Length: 366 bp

>AT43072 NZ_AP027156:4779020-4779385 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTGCCATACCGGAGAATCCCCCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTAGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGTTGATGCTCACCAGCGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCAGACTGAGCCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3U1V6G9

Antitoxin

Source ID Structure
AlphaFold DB A0A3U1V6I4

References