Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4598795..4599292 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUD66_RS22345 Protein ID WP_001173343.1
Coordinates 4599119..4599292 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUD66_RS22340 Protein ID WP_000398619.1
Coordinates 4598795..4599091 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS22320 (4595526) 4595526..4596245 - 720 WP_000611288.1 two-component system response regulator DcuR -
QUD66_RS22325 (4596242) 4596242..4597873 - 1632 WP_001216486.1 sensor histidine kinase -
QUD66_RS22330 (4598054) 4598054..4598284 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QUD66_RS22335 (4598296) 4598296..4598568 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUD66_RS22340 (4598795) 4598795..4599091 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUD66_RS22345 (4599119) 4599119..4599292 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUD66_RS22350 (4599411) 4599411..4600928 - 1518 WP_001295087.1 lysine--tRNA ligase -
QUD66_RS22355 (4601165) 4601165..4602622 - 1458 WP_244058860.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43070 WP_001173343.1 NZ_AP027156:4599119-4599292 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43070 NZ_AP027156:4599119-4599292 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43070 WP_000398619.1 NZ_AP027156:4598795-4599091 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43070 NZ_AP027156:4598795-4599091 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References