Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3860886..3861472 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag QUD66_RS18965 Protein ID WP_042014162.1
Coordinates 3861242..3861472 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUD66_RS18960 Protein ID WP_000593555.1
Coordinates 3860886..3861245 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS18935 (3857018) 3857018..3857962 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
QUD66_RS18940 (3857959) 3857959..3858792 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUD66_RS18945 (3858792) 3858792..3859556 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUD66_RS18950 (3859553) 3859553..3860359 + 807 WP_000173667.1 nickel import ATP-binding protein NikE -
QUD66_RS18955 (3860365) 3860365..3860766 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUD66_RS18960 (3860886) 3860886..3861245 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUD66_RS18965 (3861242) 3861242..3861472 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
QUD66_RS18970 (3861576) 3861576..3862700 - 1125 WP_001314210.1 ABC transporter permease -
QUD66_RS18975 (3862700) 3862700..3865435 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T43068 WP_042014162.1 NZ_AP027156:c3861472-3861242 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T43068 NZ_AP027156:c3861472-3861242 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43068 WP_000593555.1 NZ_AP027156:c3861245-3860886 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43068 NZ_AP027156:c3861245-3860886 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References