Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3267913..3268567 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QUD66_RS15950 Protein ID WP_000244781.1
Coordinates 3267913..3268320 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUD66_RS15955 Protein ID WP_000354046.1
Coordinates 3268301..3268567 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS15930 (3263870) 3263870..3265603 - 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -
QUD66_RS15935 (3265609) 3265609..3266319 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUD66_RS15940 (3266344) 3266344..3267240 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUD66_RS15945 (3267352) 3267352..3267873 + 522 WP_001055874.1 flavodoxin FldB -
QUD66_RS15950 (3267913) 3267913..3268320 - 408 WP_000244781.1 protein YgfX Toxin
QUD66_RS15955 (3268301) 3268301..3268567 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUD66_RS15960 (3268810) 3268810..3269790 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
QUD66_RS15965 (3269986) 3269986..3270645 - 660 WP_000250274.1 hemolysin III family protein -
QUD66_RS15970 (3270809) 3270809..3271120 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QUD66_RS15975 (3271165) 3271165..3272598 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
QUD66_RS15980 (3272655) 3272655..3273398 - 744 WP_000951961.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T43065 WP_000244781.1 NZ_AP027156:c3268320-3267913 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43065 NZ_AP027156:c3268320-3267913 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43065 WP_000354046.1 NZ_AP027156:c3268567-3268301 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43065 NZ_AP027156:c3268567-3268301 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References