Detailed information of TA system TA043063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2829827..2830452
• Replicon: chromosome
• Accession: NZ_AP027156
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUD66_RS13865
• Protein ID: WP_000911330.1
• Coordinates: 2829827..2830225 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QUD66_RS13870
• Protein ID: WP_000450524.1
• Coordinates: 2830225..2830452 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS13845 (2825705)	2825705..2825905	+	201	WP_000383836.1	YpfN family protein	-
QUD66_RS13850 (2826015)	2826015..2826713	-	699	WP_000679823.1	esterase	-
QUD66_RS13855 (2826787)	2826787..2828802	-	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
QUD66_RS13860 (2828817)	2828817..2829680	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QUD66_RS13865 (2829827)	2829827..2830225	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUD66_RS13870 (2830225)	2830225..2830452	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUD66_RS13875 (2830606)	2830606..2831319	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QUD66_RS13880 (2831532)	2831532..2832566	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QUD66_RS13885 (2832583)	2832583..2833461	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QUD66_RS13890 (2833607)	2833607..2834179	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QUD66_RS13895 (2834179)	2834179..2834649	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43063 WP_000911330.1 NZ_AP027156:c2830225-2829827 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43063 NZ_AP027156:c2830225-2829827 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43063 WP_000450524.1 NZ_AP027156:c2830452-2830225 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43063 NZ_AP027156:c2830452-2830225 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33