Detailed information of TA system TA043046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 454727..455564
• Replicon: chromosome
• Accession: NZ_AP027156
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUD66_RS02085
• Protein ID: WP_000227784.1
• Coordinates: 454727..455269 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUD66_RS02090
• Protein ID: WP_001297137.1
• Coordinates: 455253..455564 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS02065 (450266)	450266..451177	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QUD66_RS02070 (451345)	451345..451836	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUD66_RS02075 (451964)	451964..453328	-	1365	WP_097473795.1	MFS transporter	-
QUD66_RS02080 (453736)	453736..454671	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUD66_RS02085 (454727)	454727..455269	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUD66_RS02090 (455253)	455253..455564	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUD66_RS02095 (455749)	455749..456639	-	891	WP_000971336.1	heme o synthase	-
QUD66_RS02100 (456651)	456651..456980	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUD66_RS02105 (456980)	456980..457594	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUD66_RS02110 (457584)	457584..459575	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUD66_RS02115 (459597)	459597..460544	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43046 WP_000227784.1 NZ_AP027156:c455269-454727 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43046 NZ_AP027156:c455269-454727 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43046 WP_001297137.1 NZ_AP027156:c455564-455253 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43046 NZ_AP027156:c455564-455253 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACACTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3