Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 19790..20451 Replicon plasmid pDSM21852-1
Accession NZ_AP027150
Organism Methylocystis bryophila strain DSM 21852

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag QMG80_RS21040 Protein ID WP_281926536.1
Coordinates 20023..20451 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag QMG80_RS21035 Protein ID WP_085773936.1
Coordinates 19790..20026 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG80_RS21020 (DSM21852_42200) 15786..17009 + 1224 WP_085773938.1 plasmid partitioning protein RepA -
QMG80_RS21025 (DSM21852_42210) 17006..17965 + 960 WP_085774007.1 plasmid partitioning protein RepB -
QMG80_RS21030 (DSM21852_42220) 18184..19518 + 1335 WP_085773937.1 plasmid replication protein RepC -
QMG80_RS21035 (DSM21852_42230) 19790..20026 + 237 WP_085773936.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QMG80_RS21040 (DSM21852_42240) 20023..20451 + 429 WP_281926536.1 type II toxin-antitoxin system VapC family toxin Toxin
QMG80_RS21045 (DSM21852_42250) 20490..21623 - 1134 WP_085773934.1 tyrosine-type recombinase/integrase -
QMG80_RS21050 (DSM21852_42260) 21858..22808 + 951 WP_158658929.1 DUF1403 family protein -
QMG80_RS21055 (DSM21852_42270) 22815..23519 + 705 WP_085773932.1 SMC-Scp complex subunit ScpB -
QMG80_RS21060 (DSM21852_42280) 23812..24117 + 306 WP_085773931.1 type II toxin-antitoxin system MqsR family toxin -
QMG80_RS21065 (DSM21852_42290) 24120..24539 + 420 WP_085773930.1 type II toxin-antitoxin system MqsA family antitoxin -
QMG80_RS21070 (DSM21852_42300) 24659..24997 + 339 WP_210190845.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..174578 174578


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-128)

Antitoxin

Antitoxin VapB-like

(2-75)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15390.49 Da        Isoelectric Point: 4.5070

>T43044 WP_281926536.1 NZ_AP027150:20023-20451 [Methylocystis bryophila]
MMFVDTSAIVAILCGEDDAAELAGRLASAERRFTSPVVRLEACMVLALKLDITPAQANALFEDFLDEAHVSITPINDKIG
VLAVACFESYGKGRHPARLNLGDCLSYACAKAYRSALLFKEDDFSQTDVETTSARARNDPQS

Download         Length: 429 bp

>T43044 NZ_AP027150:20023-20451 [Methylocystis bryophila]
ATGATGTTCGTGGACACCTCCGCGATCGTAGCGATCCTCTGCGGCGAGGATGACGCTGCCGAGCTTGCGGGACGCCTCGC
GTCGGCGGAGCGGCGTTTTACGTCGCCGGTCGTCCGGCTGGAGGCGTGCATGGTGCTGGCGTTAAAGCTCGACATCACGC
CGGCGCAGGCAAACGCTCTGTTCGAGGATTTTCTCGACGAGGCGCATGTCTCGATCACGCCGATCAATGACAAGATTGGC
GTTCTCGCCGTCGCCTGCTTCGAGAGCTACGGCAAGGGACGGCATCCGGCGCGATTGAATCTCGGCGACTGCCTCTCCTA
TGCGTGCGCCAAGGCCTACCGCTCGGCGCTACTGTTCAAGGAAGACGATTTTTCGCAAACAGACGTCGAAACGACGAGCG
CGCGGGCGCGAAACGACCCGCAAAGCTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8917.49 Da        Isoelectric Point: 10.8628

>AT43044 WP_085773936.1 NZ_AP027150:19790-20026 [Methylocystis bryophila]
MLSIRDPRAAELAKLLAARRKTTMTEAIIVALENELKRERERVPLPERLARLAVKARKLAGPKGRDVPKEELDEFWGQ

Download         Length: 237 bp

>AT43044 NZ_AP027150:19790-20026 [Methylocystis bryophila]
ATGCTCAGCATACGGGACCCGCGAGCGGCAGAGCTCGCAAAGCTGCTCGCCGCTCGGCGCAAAACCACGATGACCGAAGC
CATTATCGTGGCGTTGGAGAATGAGTTGAAACGGGAGCGCGAGCGGGTTCCGCTGCCCGAGCGACTTGCGCGCTTGGCCG
TAAAAGCTCGGAAGCTCGCCGGGCCAAAGGGCCGTGACGTCCCCAAGGAAGAACTCGACGAATTCTGGGGCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References