Detailed information of TA system TA043043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 4363633..4364234
• Replicon: chromosome
• Accession: NZ_AP027149
• Organism: Methylocystis bryophila strain DSM 21852

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QMG80_RS20225
• Protein ID: WP_245300099.1
• Coordinates: 4363633..4363902 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QMG80_RS20230
• Protein ID: WP_085770810.1
• Coordinates: 4363899..4364234 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG80_RS20205 (DSM21852_40540)	4358978..4360114	-	1137	WP_085773548.1	alanine racemase	-
QMG80_RS20210 (DSM21852_40550)	4360272..4360535	+	264	WP_199769037.1	type II toxin-antitoxin system ParD family antitoxin	-
QMG80_RS20215 (DSM21852_40560)	4360532..4360912	+	381	WP_158658624.1	type II toxin-antitoxin system RelE/ParE family toxin	-
QMG80_RS20220 (DSM21852_40570)	4361018..4362718	+	1701	WP_085770808.1	lysine--tRNA ligase	-
QMG80_RS20225	4363633..4363902	+	270	WP_245300099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMG80_RS20230 (DSM21852_40600)	4363899..4364234	+	336	WP_085770810.1	putative addiction module antidote protein	Antitoxin
QMG80_RS20235 (DSM21852_40610)	4364295..4365599	-	1305	WP_085773550.1	dihydroorotase	-
QMG80_RS20240 (DSM21852_40620)	4365744..4366016	+	273	WP_085773551.1	type II toxin-antitoxin system ParD family antitoxin	-
QMG80_RS20245 (DSM21852_40630)	4366013..4366300	+	288	WP_085770811.1	type II toxin-antitoxin system RelE/ParE family toxin	-
QMG80_RS20250 (DSM21852_40640)	4366565..4366759	+	195	WP_085770812.1	hypothetical protein	-
QMG80_RS20255 (DSM21852_40650)	4366783..4367643	-	861	WP_085770813.1	enoyl-ACP reductase FabI	-
QMG80_RS20260 (DSM21852_40660)	4368063..4368404	-	342	WP_085770815.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-84)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 9846.43 Da
• Isoelectric Point: 10.1390

>T43043 WP_245300099.1 NZ_AP027149:4363633-4363902 [Methylocystis bryophila]
VAVLSPGPQGKARILARLRSATFGNFGDCASVGEGVFEMRLHFGPGYRVYYVRAEKPIYLLLIGGDKSTQKMDIVRAKEM
ARAIEETNR

Nucleotide

• Length: 270 bp

>T43043 NZ_AP027149:4363633-4363902 [Methylocystis bryophila]
GTGGCTGTCCTCTCTCCTGGACCACAAGGGAAGGCCAGAATTCTGGCGCGCTTGCGAAGCGCCACGTTCGGCAATTTCGG
CGATTGCGCTTCGGTGGGGGAAGGCGTTTTCGAAATGCGCCTCCACTTTGGACCCGGCTACCGTGTCTATTATGTGCGTG
CGGAGAAACCGATCTATCTCCTGCTGATCGGAGGCGACAAATCCACGCAAAAGATGGACATAGTCCGCGCCAAAGAGATG
GCGCGAGCGATCGAGGAGACGAATCGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 11346.67 Da
• Isoelectric Point: 4.4141

>AT43043 WP_085770810.1 NZ_AP027149:4363899-4364234 [Methylocystis bryophila]
MTTTFAPFDAADYLDSDATIAEYLTAAAEDPNSDVFLAALGDVAKARGMARIAKDTGLGRESLYKALSAGAHPRFETVNA
VLQALGVKIAIVPAGPGQDDSPAAGAADRHA

Nucleotide

• Length: 336 bp

>AT43043 NZ_AP027149:4363899-4364234 [Methylocystis bryophila]
ATGACGACCACCTTTGCCCCCTTCGACGCCGCGGATTATCTCGATAGCGACGCGACGATCGCCGAATATCTCACAGCCGC
CGCCGAGGACCCAAACAGCGATGTCTTCCTCGCCGCGCTCGGCGACGTCGCCAAAGCGCGCGGCATGGCCCGGATCGCCA
AGGACACCGGCCTCGGCCGAGAAAGCCTTTACAAGGCGCTCAGCGCCGGCGCCCATCCGCGCTTTGAAACCGTGAATGCG
GTTTTGCAGGCTCTCGGTGTGAAAATCGCGATCGTCCCCGCGGGACCCGGACAGGACGACTCGCCGGCTGCCGGCGCGGC
AGACCGCCACGCATGA