Detailed information of TA system TA043035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3438949..3439540
• Replicon: chromosome
• Accession: NZ_AP027142
• Organism: Methylocystis iwaonis strain SS37A-Re

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: QMG84_RS16370
• Protein ID: WP_281929221.1
• Coordinates: 3439208..3439540 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: QMG84_RS16365
• Protein ID: WP_281929219.1
• Coordinates: 3438949..3439206 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG84_RS16340 (SS37A_32380)	3434276..3434524	+	249	WP_281929209.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
QMG84_RS16345 (SS37A_32390)	3434491..3434904	+	414	WP_281929211.1	type II toxin-antitoxin system VapC family toxin	-
QMG84_RS16350 (SS37A_32400)	3435019..3435723	+	705	WP_281929213.1	preprotein translocase subunit SecG	-
QMG84_RS16355 (SS37A_32410)	3435833..3437461	+	1629	WP_281929215.1	CTP synthase	-
QMG84_RS16360 (SS37A_32420)	3437529..3438701	-	1173	WP_281929217.1	IS481 family transposase	-
QMG84_RS16365 (SS37A_32430)	3438949..3439206	+	258	WP_281929219.1	antitoxin	Antitoxin
QMG84_RS16370 (SS37A_32440)	3439208..3439540	+	333	WP_281929221.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QMG84_RS16375 (SS37A_32450)	3439800..3440033	+	234	WP_281929223.1	DUF433 domain-containing protein	-
QMG84_RS16380 (SS37A_32460)	3440035..3440391	+	357	WP_281929224.1	DUF5615 family PIN-like protein	-
QMG84_RS16385 (SS37A_32470)	3440411..3440614	-	204	WP_281932049.1	ribbon-helix-helix domain-containing protein	-
QMG84_RS16390 (SS37A_32480)	3440713..3440895	-	183	WP_281929225.1	DUF4169 family protein	-
QMG84_RS16395 (SS37A_32490)	3440895..3441461	-	567	WP_281929226.1	ClpXP protease specificity-enhancing factor SspB	-
QMG84_RS16405 (SS37A_32500)	3442161..3443045	+	885	WP_202071357.1	succinate--CoA ligase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3437529..3438701	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11571.28 Da
• Isoelectric Point: 9.1678

>T43035 WP_281929221.1 NZ_AP027142:3439208-3439540 [Methylocystis iwaonis]
MERGDIYLVSLDPTSGHEQRGARPVLIVSATAFNKITKTPIVLPITSSGNFARTAGFAVSLSGAGTQTTGVIRCDQPRAL
DLGARRGRKLESAPPEIVDEALAKLAAILE

Nucleotide

• Length: 333 bp

>T43035 NZ_AP027142:3439208-3439540 [Methylocystis iwaonis]
ATGGAGCGCGGCGACATCTACCTCGTCTCGCTCGATCCGACCTCGGGGCACGAGCAACGGGGCGCGCGGCCGGTGCTCAT
CGTCTCGGCGACGGCCTTCAACAAAATAACGAAAACGCCCATCGTCCTGCCGATTACCAGCAGCGGCAATTTCGCGCGCA
CAGCCGGCTTTGCAGTGTCTCTCTCGGGCGCAGGCACGCAGACGACCGGCGTCATCCGTTGCGACCAGCCGCGCGCCCTC
GACCTCGGCGCACGCCGTGGCCGCAAGCTCGAAAGCGCACCGCCGGAAATCGTCGACGAAGCGCTCGCCAAGCTCGCCGC
CATTTTGGAATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8846.18 Da
• Isoelectric Point: 4.4472

>AT43035 WP_281929219.1 NZ_AP027142:3438949-3439206 [Methylocystis iwaonis]
MPTTTLRKVGGSVMLALPPAILDLLNLKAGATVGVSVEDGKLVIAPASKPRYTLGDLLAQCDAQGESSEEDRAWLGDKPQ
GSELL

Nucleotide

• Length: 258 bp

>AT43035 NZ_AP027142:3438949-3439206 [Methylocystis iwaonis]
ATGCCCACAACCACTCTTCGGAAAGTCGGCGGGTCAGTCATGTTGGCTCTGCCGCCTGCCATTCTCGACCTCCTTAACTT
GAAGGCCGGGGCGACGGTTGGCGTCTCCGTCGAAGACGGCAAGCTTGTTATCGCGCCAGCGTCAAAGCCGCGCTACACGC
TCGGCGACCTTCTCGCCCAATGCGACGCCCAGGGCGAATCTTCCGAGGAAGATCGCGCCTGGCTCGGTGATAAGCCGCAA
GGCTCGGAGCTGCTCTGA