43029

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 80148..81285 Replicon plasmid pJARB-HU0796_01
Accession NZ_AP027137
Organism Enterococcus faecalis strain JARB-HU0796

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag QUD94_RS13500 Protein ID WP_002332783.1
Coordinates 80148..81011 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag QUD94_RS13505 Protein ID WP_002326825.1
Coordinates 81013..81285 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD94_RS13470 (JARBHU0796_26360) 76474..77211 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
QUD94_RS13475 77336..77419 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
QUD94_RS13480 77468..77551 - 84 Protein_84 MLS leader peptide -
QUD94_RS13485 (JARBHU0796_26370) 77874..78554 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
QUD94_RS13490 (JARBHU0796_26380) 78588..79094 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
QUD94_RS13495 (JARBHU0796_26390) 79391..79708 - 318 WP_002326830.1 hypothetical protein -
QUD94_RS13500 (JARBHU0796_26400) 80148..81011 - 864 WP_002332783.1 zeta toxin family protein Toxin
QUD94_RS13505 (JARBHU0796_26410) 81013..81285 - 273 WP_002326825.1 antitoxin Antitoxin
QUD94_RS13510 (JARBHU0796_26420) 81303..81518 - 216 WP_001835296.1 peptide-binding protein -
QUD94_RS13515 (JARBHU0796_26430) 81610..82506 - 897 WP_002326827.1 ParA family protein -
QUD94_RS13520 82609..82869 - 261 Protein_92 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
QUD94_RS13525 (JARBHU0796_26440) 83039..83776 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
QUD94_RS13530 83901..83984 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
QUD94_RS13535 84033..84116 - 84 Protein_95 MLS leader peptide -
QUD94_RS13540 (JARBHU0796_26450) 84416..84787 - 372 WP_002358205.1 hypothetical protein -
QUD94_RS13545 (JARBHU0796_26460) 84780..85625 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / dfrG - 1..85996 85996
- inside IS/Tn erm(B) / dfrG - 76474..83776 7302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T43029 WP_002332783.1 NZ_AP027137:c81011-80148 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T43029 NZ_AP027137:c81011-80148 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT43029 WP_002326825.1 NZ_AP027137:c81285-81013 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT43029 NZ_AP027137:c81285-81013 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References