Detailed information of TA system TA043028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 69867..70438
• Replicon: plasmid pJARB-HU0796_01
• Accession: NZ_AP027137
• Organism: Enterococcus faecalis strain JARB-HU0796

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S4H3R9
• Locus tag: QUD94_RS13400
• Protein ID: WP_010784114.1
• Coordinates: 69867..70208 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: QUD94_RS13405
• Protein ID: WP_002362431.1
• Coordinates: 70208..70438 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD94_RS13380 (JARBHU0796_26180)	65266..66549	-	1284	WP_002405123.1	hypothetical protein	-
QUD94_RS13390 (JARBHU0796_26210)	67950..68552	-	603	WP_002362434.1	Fic family protein	-
QUD94_RS13395 (JARBHU0796_26220)	68817..69755	-	939	WP_002362433.1	hypothetical protein	-
QUD94_RS13400 (JARBHU0796_26230)	69867..70208	-	342	WP_010784114.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUD94_RS13405 (JARBHU0796_26240)	70208..70438	-	231	WP_002362431.1	hypothetical protein	Antitoxin
QUD94_RS13410 (JARBHU0796_26250)	70642..71262	+	621	WP_002367784.1	recombinase family protein	-
QUD94_RS13415 (JARBHU0796_26260)	71252..71566	+	315	WP_002367785.1	hypothetical protein	-
QUD94_RS13420 (JARBHU0796_26270)	71560..71766	+	207	WP_002367786.1	hypothetical protein	-
QUD94_RS13425 (JARBHU0796_26280)	71926..72120	+	195	WP_002367787.1	hypothetical protein	-
QUD94_RS13430 (JARBHU0796_26290)	72132..72323	+	192	WP_002367788.1	hypothetical protein	-
QUD94_RS13435 (JARBHU0796_26300)	72493..72708	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
QUD94_RS13440 (JARBHU0796_26310)	72709..73050	+	342	WP_002367792.1	bacteriocin immunity protein	-
QUD94_RS13445 (JARBHU0796_26320)	73466..73984	+	519	WP_002367793.1	hypothetical protein	-
QUD94_RS13450 (JARBHU0796_26330)	73932..74147	+	216	WP_002415356.1	hypothetical protein	-
QUD94_RS13455	74239..74325	+	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
QUD94_RS13460 (JARBHU0796_26340)	74582..74878	+	297	WP_002367795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cat / tet(L) / tet(M) / erm(B) / dfrG	-	1..85996	85996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13270.47 Da
• Isoelectric Point: 7.9750

>T43028 WP_010784114.1 NZ_AP027137:c70208-69867 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T43028 NZ_AP027137:c70208-69867 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT43028 WP_002362431.1 NZ_AP027137:c70438-70208 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT43028 NZ_AP027137:c70438-70208 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: S4H3R9

Antitoxin

• AlphaFold DB: R3KHK9