Detailed information of TA system TA043020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2618284..2618855
• Replicon: chromosome
• Accession: NZ_AP027136
• Organism: Enterococcus faecalis strain JARB-HU0796

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QUD94_RS12590
• Protein ID: WP_010710856.1
• Coordinates: 2618284..2618625 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: QUD94_RS12595
• Protein ID: WP_002354773.1
• Coordinates: 2618625..2618855 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD94_RS12585 (2614299)	2614299..2617913	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
QUD94_RS12590 (2618284)	2618284..2618625	-	342	WP_010710856.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUD94_RS12595 (2618625)	2618625..2618855	-	231	WP_002354773.1	hypothetical protein	Antitoxin
QUD94_RS12600 (2619186)	2619186..2619401	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
QUD94_RS12605 (2619540)	2619540..2620532	+	993	WP_002394775.1	biotin--[acetyl-CoA-carboxylase] ligase	-
QUD94_RS12610 (2620699)	2620699..2621337	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
QUD94_RS12615 (2622022)	2622022..2623638	+	1617	WP_010710855.1	phosphatase PAP2/LCP family protein	-
- (2623722)	2623722..2623797	-	76	NuclAT_6	-	-
- (2623695)	2623695..2623811	-	117	NuclAT_8	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13177.47 Da
• Isoelectric Point: 9.3988

>T43020 WP_010710856.1 NZ_AP027136:c2618625-2618284 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPIISTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T43020 NZ_AP027136:c2618625-2618284 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
TTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT43020 WP_002354773.1 NZ_AP027136:c2618855-2618625 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT43020 NZ_AP027136:c2618855-2618625 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812