Detailed information of TA system TA043002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2120140..2120669
• Replicon: chromosome
• Accession: NZ_AP027135
• Organism: Staphylococcus aureus strain JP089

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QMM73_RS10570
• Protein ID: WP_000621175.1
• Coordinates: 2120140..2120502 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: QMM73_RS10575
• Protein ID: WP_000948331.1
• Coordinates: 2120499..2120669 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMM73_RS10550 (2117119)	2117119..2117889	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
QMM73_RS10555 (2117864)	2117864..2118343	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
QMM73_RS10560 (2118345)	2118345..2118671	-	327	WP_001052491.1	anti-sigma factor antagonist	-
QMM73_RS10565 (2118790)	2118790..2119791	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
QMM73_RS10570 (2120140)	2120140..2120502	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QMM73_RS10575 (2120499)	2120499..2120669	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
QMM73_RS10580 (2120754)	2120754..2121902	-	1149	WP_281961710.1	alanine racemase	-
QMM73_RS10585 (2121968)	2121968..2122327	-	360	WP_061838820.1	holo-ACP synthase	-
QMM73_RS10590 (2122331)	2122331..2122822	-	492	WP_072507282.1	PH domain-containing protein	-
QMM73_RS10595 (2122809)	2122809..2124392	-	1584	WP_061838822.1	PH domain-containing protein	-
QMM73_RS10600 (2124385)	2124385..2124864	-	480	WP_001287078.1	hypothetical protein	-
QMM73_RS10605 (2125073)	2125073..2125633	-	561	WP_061838823.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T43002 WP_000621175.1 NZ_AP027135:c2120502-2120140 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T43002 NZ_AP027135:c2120502-2120140 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTAGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT43002 WP_000948331.1 NZ_AP027135:c2120669-2120499 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT43002 NZ_AP027135:c2120669-2120499 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1