42978

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 89126..89706 Replicon plasmid pTEGF4
Accession NZ_AP027057
Organism Limnohabitans sp. TEGF004

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag LINBF2_RS13310 Protein ID WP_281891359.1
Coordinates 89380..89706 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag LINBF2_RS13305 Protein ID WP_281891358.1
Coordinates 89126..89383 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LINBF2_RS13290 (LTEGF4_26280) 85311..86630 + 1320 WP_281891477.1 homoserine dehydrogenase -
LINBF2_RS13295 (LTEGF4_26300) 87476..88594 + 1119 WP_281891356.1 ATP-binding protein -
LINBF2_RS13300 (LTEGF4_26310) 88613..89011 + 399 WP_281891357.1 H-NS histone family protein -
LINBF2_RS13305 (LTEGF4_26320) 89126..89383 + 258 WP_281891358.1 antitoxin Antitoxin
LINBF2_RS13310 (LTEGF4_26330) 89380..89706 + 327 WP_281891359.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LINBF2_RS13315 (LTEGF4_26340) 89823..90131 + 309 WP_281891360.1 putative addiction module antidote protein -
LINBF2_RS13320 (LTEGF4_26350) 90300..90608 + 309 WP_281891478.1 HigA family addiction module antitoxin -
LINBF2_RS13325 (LTEGF4_26370) 91295..91657 + 363 WP_281891361.1 hypothetical protein -
LINBF2_RS13330 (LTEGF4_26380) 91675..91995 + 321 WP_281891362.1 TrbC/VirB2 family protein -
LINBF2_RS13335 92008..92328 + 321 WP_281891363.1 VirB3 family type IV secretion system protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..148919 148919


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11563.49 Da        Isoelectric Point: 7.1660

>T42978 WP_281891359.1 NZ_AP027057:89380-89706 [Limnohabitans sp. TEGF004]
MKRGDIYLVSLDPTAGHEQSGSLPVLVVSPAEFNDATKLPVILPITNGGEFARRLGFAVPVTGIKTTGVVRCDQPRVIDL
AARHARKVDTLPALLMDEVLAKVATVFE

Download         Length: 327 bp

>T42978 NZ_AP027057:89380-89706 [Limnohabitans sp. TEGF004]
ATGAAGCGAGGAGACATCTATCTTGTATCGCTCGATCCGACCGCCGGTCACGAACAAAGCGGCAGCCTCCCCGTCCTGGT
CGTATCGCCTGCCGAGTTCAACGATGCCACGAAATTACCAGTGATCTTGCCCATCACCAACGGCGGTGAGTTCGCGCGCC
GGCTTGGCTTCGCCGTGCCCGTAACCGGCATCAAGACGACCGGCGTCGTGCGCTGTGACCAGCCGCGCGTAATCGATCTG
GCCGCCCGTCATGCCCGTAAGGTGGATACGTTGCCGGCGCTACTCATGGATGAAGTGCTGGCAAAGGTCGCCACGGTCTT
CGAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9204.57 Da        Isoelectric Point: 5.5143

>AT42978 WP_281891358.1 NZ_AP027057:89126-89383 [Limnohabitans sp. TEGF004]
MHTTNLRKVGGSIMLAVPPAFLDQLHLQAGATVGLAVDHGCLVVNPRPRPRYTLAELLATSDYSQPQPTEEREWIDAPAM
GGELL

Download         Length: 258 bp

>AT42978 NZ_AP027057:89126-89383 [Limnohabitans sp. TEGF004]
ATGCATACAACCAATCTTCGGAAGGTCGGCGGCTCAATCATGTTGGCCGTTCCGCCGGCGTTCCTTGACCAACTGCACCT
GCAAGCTGGTGCGACAGTCGGTCTCGCAGTCGATCACGGCTGCTTGGTGGTCAATCCCCGTCCACGGCCACGCTACACGC
TCGCAGAGTTGCTGGCCACGTCGGACTATTCGCAGCCGCAACCGACCGAGGAACGCGAATGGATCGATGCGCCTGCCATG
GGTGGTGAACTGTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References