Detailed information of TA system TA042977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParE-CopA/ParE-RHH
• Location: 80728..81296
• Replicon: plasmid pTEGF4
• Accession: NZ_AP027057
• Organism: Limnohabitans sp. TEGF004

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LINBF2_RS13270
• Protein ID: WP_281891354.1
• Coordinates: 81006..81296 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: copA
• Uniprot ID: -
• Locus tag: LINBF2_RS13265
• Protein ID: WP_281891353.1
• Coordinates: 80728..81018 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LINBF2_RS13235 (LTEGF4_26170)	76370..76915	-	546	WP_281891350.1	porin family protein	-
LINBF2_RS13240 (LTEGF4_26180)	77288..77503	+	216	WP_281891351.1	hypothetical protein	-
LINBF2_RS13245	78334..78801	-	468	Protein_75	hypothetical protein	-
LINBF2_RS13250 (LTEGF4_26190)	78805..79182	+	378	WP_281891352.1	hypothetical protein	-
LINBF2_RS13255 (LTEGF4_26210)	79237..80002	-	766	Protein_77	DUF4158 domain-containing protein	-
LINBF2_RS13260	80088..80549	-	462	Protein_78	recombinase family protein	-
LINBF2_RS13265 (LTEGF4_26230)	80728..81018	+	291	WP_281891353.1	ribbon-helix-helix protein, CopG family	Antitoxin
LINBF2_RS13270 (LTEGF4_26240)	81006..81296	+	291	WP_281891354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LINBF2_RS13275 (LTEGF4_26250)	81302..81837	+	536	Protein_81	transposase	-
LINBF2_RS13280 (LTEGF4_26260)	82119..82639	-	521	Protein_82	winged helix DNA-binding protein	-
LINBF2_RS13285 (LTEGF4_26270)	82816..84378	-	1563	WP_281891355.1	DUF5666 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..148919	148919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-44)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10938.63 Da
• Isoelectric Point: 10.1326

>T42977 WP_281891354.1 NZ_AP027057:81006-81296 [Limnohabitans sp. TEGF004]
MSRLIWSQPALLNVQRLYRFLAAKNLDAAKRAVKAIRHGVKVLGQQPGVGRPVEDMPDEFREWIIDFGDSGYVARYRIDS
DAVTILAVRHQKEVGY

Nucleotide

• Length: 291 bp

>T42977 NZ_AP027057:81006-81296 [Limnohabitans sp. TEGF004]
ATGTCACGTCTGATCTGGTCACAACCGGCGCTGCTCAATGTGCAGCGCCTCTATCGATTCCTGGCGGCAAAGAACCTTGA
CGCTGCAAAGCGCGCGGTGAAGGCCATCCGCCACGGCGTGAAGGTGCTGGGGCAGCAGCCCGGCGTCGGCCGCCCCGTCG
AGGACATGCCAGACGAGTTTCGGGAGTGGATCATTGACTTCGGCGATAGCGGCTACGTGGCCCGCTACCGCATCGACTCG
GATGCCGTCACGATCCTCGCGGTTCGGCACCAGAAAGAGGTGGGCTACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11243.80 Da
• Isoelectric Point: 6.8726

>AT42977 WP_281891353.1 NZ_AP027057:80728-81018 [Limnohabitans sp. TEGF004]
MTTAAVRPVAIKIDEDIKVRVKRLADARHRTSHWLMREAITQYVFREEKREAFRQDTLKAWEEYRTTGLHVTVDEADAWL
AQLEQGKDIEPPACHV

Nucleotide

• Length: 291 bp

>AT42977 NZ_AP027057:80728-81018 [Limnohabitans sp. TEGF004]
ATGACCACTGCTGCCGTTCGCCCTGTGGCGATCAAGATCGACGAGGACATCAAGGTGCGAGTGAAGCGCCTGGCTGACGC
ACGTCACCGCACGTCGCACTGGTTGATGCGCGAGGCGATCACCCAGTACGTTTTTCGCGAGGAAAAGCGCGAGGCCTTCC
GCCAGGACACGCTCAAGGCATGGGAAGAGTACCGCACCACCGGACTGCATGTGACCGTCGATGAGGCCGATGCTTGGCTT
GCCCAGCTCGAACAGGGCAAGGATATAGAACCTCCGGCATGTCACGTCTGA