Detailed information of TA system TA042975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParE-CopA/ParE-RHH
• Location: 9913..10481
• Replicon: plasmid pTEGF4
• Accession: NZ_AP027057
• Organism: Limnohabitans sp. TEGF004

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LINBF2_RS12930
• Protein ID: WP_281891354.1
• Coordinates: 10191..10481 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: copA
• Uniprot ID: -
• Locus tag: LINBF2_RS12925
• Protein ID: WP_281891353.1
• Coordinates: 9913..10203 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LINBF2_RS12895 (LTEGF4_25490)	6338..6691	+	354	WP_281891427.1	RidA family protein	-
LINBF2_RS12900 (LTEGF4_25500)	6911..7207	+	297	WP_281891428.1	HipA domain-containing protein	-
LINBF2_RS12905 (LTEGF4_25510)	7519..7986	-	468	Protein_7	hypothetical protein	-
LINBF2_RS12910 (LTEGF4_25520)	7990..8367	+	378	WP_281891352.1	hypothetical protein	-
LINBF2_RS12915 (LTEGF4_25540)	8422..9187	-	766	Protein_9	DUF4158 domain-containing protein	-
LINBF2_RS12920	9273..9734	-	462	Protein_10	recombinase family protein	-
LINBF2_RS12925 (LTEGF4_25560)	9913..10203	+	291	WP_281891353.1	ribbon-helix-helix protein, CopG family	Antitoxin
LINBF2_RS12930 (LTEGF4_25570)	10191..10481	+	291	WP_281891354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LINBF2_RS12935 (LTEGF4_25580)	10487..11022	+	536	Protein_13	transposase	-
LINBF2_RS12940 (LTEGF4_25590)	12167..12559	+	393	WP_281891429.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LINBF2_RS12945 (LTEGF4_25600)	12564..12911	+	348	WP_281891482.1	XRE family transcriptional regulator	-
LINBF2_RS12950 (LTEGF4_25610)	13346..13579	+	234	WP_281891430.1	type II toxin-antitoxin system VapB family antitoxin	-
LINBF2_RS12955 (LTEGF4_25620)	13579..13989	+	411	WP_281891431.1	tRNA(fMet)-specific endonuclease VapC	-
LINBF2_RS12960 (LTEGF4_25630)	14427..14846	-	420	WP_281891432.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..148919	148919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-44)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10938.63 Da
• Isoelectric Point: 10.1326

>T42975 WP_281891354.1 NZ_AP027057:10191-10481 [Limnohabitans sp. TEGF004]
MSRLIWSQPALLNVQRLYRFLAAKNLDAAKRAVKAIRHGVKVLGQQPGVGRPVEDMPDEFREWIIDFGDSGYVARYRIDS
DAVTILAVRHQKEVGY

Nucleotide

• Length: 291 bp

>T42975 NZ_AP027057:10191-10481 [Limnohabitans sp. TEGF004]
ATGTCACGTCTGATCTGGTCACAACCGGCGCTGCTCAATGTGCAGCGCCTCTATCGATTCCTGGCGGCAAAGAACCTTGA
CGCTGCAAAGCGCGCGGTGAAGGCCATCCGCCACGGCGTGAAGGTGCTGGGGCAGCAGCCCGGCGTCGGCCGCCCCGTCG
AGGACATGCCAGACGAGTTTCGGGAGTGGATCATTGACTTCGGCGATAGCGGCTACGTGGCCCGCTACCGCATCGACTCG
GATGCCGTCACGATCCTCGCGGTTCGGCACCAGAAAGAGGTGGGCTACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11243.80 Da
• Isoelectric Point: 6.8726

>AT42975 WP_281891353.1 NZ_AP027057:9913-10203 [Limnohabitans sp. TEGF004]
MTTAAVRPVAIKIDEDIKVRVKRLADARHRTSHWLMREAITQYVFREEKREAFRQDTLKAWEEYRTTGLHVTVDEADAWL
AQLEQGKDIEPPACHV

Nucleotide

• Length: 291 bp

>AT42975 NZ_AP027057:9913-10203 [Limnohabitans sp. TEGF004]
ATGACCACTGCTGCCGTTCGCCCTGTGGCGATCAAGATCGACGAGGACATCAAGGTGCGAGTGAAGCGCCTGGCTGACGC
ACGTCACCGCACGTCGCACTGGTTGATGCGCGAGGCGATCACCCAGTACGTTTTTCGCGAGGAAAAGCGCGAGGCCTTCC
GCCAGGACACGCTCAAGGCATGGGAAGAGTACCGCACCACCGGACTGCATGTGACCGTCGATGAGGCCGATGCTTGGCTT
GCCCAGCTCGAACAGGGCAAGGATATAGAACCTCCGGCATGTCACGTCTGA