Detailed information of TA system TA042950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 281303..281902
• Replicon: chromosome
• Accession: NZ_AP026974
• Organism: Polynucleobacter yangtzensis strain KF032

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QMF30_RS01520
• Protein ID: WP_281745564.1
• Coordinates: 281303..281599 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QMF30_RS01525
• Protein ID: WP_281745565.1
• Coordinates: 281600..281902 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMF30_RS01495 (PKF032_02860)	277454..278272	-	819	WP_281745561.1	cell envelope integrity protein TolA	-
QMF30_RS01500 (PKF032_02870)	278298..278717	-	420	WP_068320508.1	protein TolR	-
QMF30_RS01505 (PKF032_02880)	278754..279410	-	657	WP_068320510.1	protein TolQ	-
QMF30_RS01510 (PKF032_02890)	279487..280731	+	1245	WP_281745563.1	serine hydroxymethyltransferase	-
QMF30_RS01515 (PKF032_02900)	280759..281205	+	447	WP_068320514.1	transcriptional regulator NrdR	-
QMF30_RS01520 (PKF032_02910)	281303..281599	+	297	WP_281745564.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMF30_RS01525 (PKF032_02920)	281600..281902	+	303	WP_281745565.1	putative addiction module antidote protein	Antitoxin
QMF30_RS01530 (PKF032_02930)	281909..282874	-	966	WP_281745567.1	GDP-mannose 4,6-dehydratase	-
QMF30_RS01535 (PKF032_02940)	282884..283549	-	666	WP_281745568.1	adenylate kinase	-
QMF30_RS01540 (PKF032_02950)	283663..284427	-	765	WP_281745570.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
QMF30_RS01545 (PKF032_02960)	284448..284627	-	180	WP_281745572.1	Trm112 family protein	-
QMF30_RS01550 (PKF032_02970)	284726..285811	-	1086	WP_281745575.1	tetraacyldisaccharide 4'-kinase	-
QMF30_RS01555 (PKF032_02980)	285860..286327	-	468	WP_281745576.1	biopolymer transporter ExbD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (12-97)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11139.97 Da
• Isoelectric Point: 10.1285

>T42950 WP_281745564.1 NZ_AP026974:281303-281599 [Polynucleobacter yangtzensis]
MYEIKKTDEFENWFSGIRDSLTRGRLLARLRKASLGSLGDIASVGGGVWEMREHFGAGYRMYYVSYRRTIILMLGGGCKS
TQTSDIKKVKKLLLVLED

Nucleotide

• Length: 297 bp

>T42950 NZ_AP026974:281303-281599 [Polynucleobacter yangtzensis]
ATGTATGAAATCAAAAAAACAGATGAATTTGAGAATTGGTTTTCTGGTATTCGCGACTCTTTAACGCGAGGCCGCCTCCT
AGCAAGACTGCGTAAGGCTTCGCTAGGGAGTCTTGGAGATATAGCTTCAGTCGGTGGTGGCGTGTGGGAAATGAGAGAGC
ATTTCGGTGCCGGCTACAGAATGTATTACGTTAGTTATCGAAGGACCATCATTCTGATGTTGGGTGGTGGATGCAAATCT
ACACAAACTTCTGATATTAAAAAGGTTAAGAAATTGCTTCTAGTCCTGGAGGATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11011.93 Da
• Isoelectric Point: 10.3001

>AT42950 WP_281745565.1 NZ_AP026974:281600-281902 [Polynucleobacter yangtzensis]
MNSAKKTKIKDLKKFDVVDYLKTEKDIAAYLSAVLEDGDPALFVAAIGDIARAKGMTEIAKKSGVTRESLYRALKVEARP
RFETVTRVVQALGMKLSIHA

Nucleotide

• Length: 303 bp

>AT42950 NZ_AP026974:281600-281902 [Polynucleobacter yangtzensis]
ATGAATAGTGCCAAAAAGACCAAAATAAAAGATTTAAAAAAATTCGATGTTGTTGACTATCTAAAGACAGAAAAAGACAT
TGCTGCTTATCTTTCCGCTGTTTTAGAGGATGGTGATCCCGCTTTGTTTGTTGCTGCTATTGGCGATATTGCCAGAGCTA
AAGGGATGACGGAGATTGCTAAGAAGAGTGGTGTTACGCGAGAGTCTCTGTATAGAGCTTTAAAAGTAGAGGCTAGACCA
CGATTTGAAACTGTGACGCGAGTGGTGCAGGCACTAGGCATGAAACTGAGTATTCACGCCTAA