Detailed information of TA system TA042944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 27173..27695
• Replicon: plasmid pEC20-C72-1-2
• Accession: NZ_AP026963
• Organism: Escherichia coli strain EC20-72C-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: OO975_RS23865
• Protein ID: WP_000220560.1
• Coordinates: 27414..27695 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: OO975_RS23860
• Protein ID: WP_000121743.1
• Coordinates: 27173..27424 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO975_RS23835 (EC2072C1_45890)	22844..23125	+	282	WP_001185482.1	hypothetical protein	-
OO975_RS23840 (EC2072C1_45900)	23150..24127	+	978	WP_024245156.1	hypothetical protein	-
OO975_RS23845 (EC2072C1_45910)	24124..24480	-	357	WP_000456533.1	DNA distortion polypeptide 3	-
OO975_RS23850	24617..25063	-	447	Protein_31	hypothetical protein	-
OO975_RS23855 (EC2072C1_45940)	25103..25939	-	837	WP_001050931.1	RepB family plasmid replication initiator protein	-
OO975_RS23860 (EC2072C1_45950)	27173..27424	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
OO975_RS23865 (EC2072C1_45960)	27414..27695	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OO975_RS23870 (EC2072C1_45980)	27770..28467	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
OO975_RS23875 (EC2072C1_45990)	28769..28963	+	195	WP_053273080.1	hypothetical protein	-
OO975_RS23880	29155..29406	-	252	Protein_37	IS200/IS605 family transposase	-
OO975_RS23885 (EC2072C1_46000)	29462..30159	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
OO975_RS23890 (EC2072C1_46010)	30280..31118	-	839	Protein_39	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
OO975_RS23895 (EC2072C1_46020)	31267..32208	+	942	Protein_40	class 1 integron integrase IntI1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(3)-IId / ant(3'')-Ia / qnrS1 / sul2 / floR / blaTEM-1B	-	1..87025	87025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T42944 WP_000220560.1 NZ_AP026963:27414-27695 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T42944 NZ_AP026963:27414-27695 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT42944 WP_000121743.1 NZ_AP026963:27173-27424 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT42944 NZ_AP026963:27173-27424 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301