Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90951..92574 Replicon plasmid pEC20-C72-1-1
Accession NZ_AP026962
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag OO975_RS22755 Protein ID WP_000088046.1
Coordinates 91249..92574 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag OO975_RS22750 Protein ID WP_024136327.1
Coordinates 90951..91244 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS22715 (EC2072C1_43660) 86021..86152 + 132 WP_000374059.1 hypothetical protein -
OO975_RS22720 (EC2072C1_43670) 86493..86774 - 282 WP_000077926.1 hypothetical protein -
OO975_RS22725 (EC2072C1_43680) 86824..87015 - 192 WP_000398480.1 hypothetical protein -
OO975_RS22730 (EC2072C1_43690) 87239..88411 + 1173 WP_000952349.1 IS21-like element IS21 family transposase -
OO975_RS22735 (EC2072C1_43700) 88411..89208 + 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
OO975_RS22740 (EC2072C1_43710) 89247..89582 - 336 WP_065311125.1 hypothetical protein -
OO975_RS22745 (EC2072C1_43720) 89955..90347 + 393 WP_000341066.1 hypothetical protein -
OO975_RS22750 (EC2072C1_43730) 90951..91244 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
OO975_RS22755 (EC2072C1_43740) 91249..92574 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
OO975_RS22760 (EC2072C1_43750) 92635..92841 + 207 WP_000134171.1 hypothetical protein -
OO975_RS22765 (EC2072C1_43760) 92943..93353 + 411 WP_000985911.1 hypothetical protein -
OO975_RS22770 (EC2072C1_43770) 93366..94181 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
OO975_RS22775 (EC2072C1_43780) 94435..94860 + 426 WP_001043843.1 hypothetical protein -
OO975_RS22780 (EC2072C1_43790) 95409..95717 + 309 WP_001224687.1 hypothetical protein -
OO975_RS22785 (EC2072C1_43800) 95733..96590 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
OO975_RS22790 (EC2072C1_43810) 96652..96855 + 204 WP_001194555.1 hypothetical protein -
OO975_RS22795 (EC2072C1_43820) 97197..97553 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / aph(3')-Ia / tet(A) / floR / qnrS1 / blaCTX-M-55 / aac(3)-IId / mph(A) / ARR-3 / dfrA14 - 1..254266 254266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T42943 WP_000088046.1 NZ_AP026962:91249-92574 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T42943 NZ_AP026962:91249-92574 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT42943 WP_024136327.1 NZ_AP026962:90951-91244 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT42943 NZ_AP026962:90951-91244 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References