42941

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3921875..3922451 Replicon chromosome
Accession NZ_AP026961
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag OO975_RS19105 Protein ID WP_001297643.1
Coordinates 3922164..3922451 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag OO975_RS19100 Protein ID WP_000063156.1
Coordinates 3921875..3922177 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS19085 (3918471) 3918471..3920621 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
OO975_RS19090 (3920671) 3920671..3920874 + 204 WP_000467859.1 YbdD/YjiX family protein -
OO975_RS19095 (3920885) 3920885..3921841 + 957 WP_001297640.1 GTPase -
OO975_RS19100 (3921875) 3921875..3922177 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
OO975_RS19105 (3922164) 3922164..3922451 - 288 WP_001297643.1 BrnT family toxin Toxin
OO975_RS19110 (3922650) 3922650..3923347 - 698 Protein_3737 IS1 family transposase -
OO975_RS19115 (3923404) 3923404..3923499 + 96 Protein_3738 dienelactone hydrolase family protein -
OO975_RS19120 (3923645) 3923645..3924877 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
OO975_RS19125 (3924918) 3924918..3926198 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T42941 WP_001297643.1 NZ_AP026961:c3922451-3922164 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T42941 NZ_AP026961:c3922451-3922164 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT42941 WP_000063156.1 NZ_AP026961:c3922177-3921875 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT42941 NZ_AP026961:c3922177-3921875 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References