Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 75047..75648 Replicon plasmid pEC20-4B-2-1
Accession NZ_AP026938
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag OO845_RS22725 Protein ID WP_001216034.1
Coordinates 75268..75648 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag OO845_RS22720 Protein ID WP_001190712.1
Coordinates 75047..75268 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS22690 (EC204B2_43380) 70156..70662 + 507 WP_000021754.1 hypothetical protein -
OO845_RS22695 (EC204B2_43390) 70857..72035 + 1179 WP_094318253.1 hypothetical protein -
OO845_RS22700 (EC204B2_43400) 72163..72393 + 231 WP_223656858.1 hypothetical protein -
OO845_RS22705 (EC204B2_43410) 73469..73825 - 357 WP_001062545.1 hypothetical protein -
OO845_RS22710 (EC204B2_43420) 73826..74233 - 408 WP_223656859.1 hypothetical protein -
OO845_RS22715 (EC204B2_43430) 74585..74974 + 390 WP_000506724.1 S24 family peptidase -
OO845_RS22720 (EC204B2_43440) 75047..75268 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OO845_RS22725 (EC204B2_43450) 75268..75648 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
OO845_RS22730 (EC204B2_43460) 75653..75832 + 180 WP_000113018.1 hypothetical protein -
OO845_RS22735 (EC204B2_43470) 75860..76903 + 1044 WP_112918711.1 DUF968 domain-containing protein -
OO845_RS22740 (EC204B2_43480) 76992..77444 + 453 WP_001326849.1 late promoter-activating protein -
OO845_RS22745 (EC204B2_43490) 77530..78723 + 1194 WP_032214401.1 hypothetical protein -
OO845_RS22750 (EC204B2_43500) 78765..80207 + 1443 WP_223656860.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / floR / dfrA14 - 1..160424 160424


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T42901 WP_001216034.1 NZ_AP026938:75268-75648 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T42901 NZ_AP026938:75268-75648 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTCGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT42901 WP_001190712.1 NZ_AP026938:75047-75268 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT42901 NZ_AP026938:75047-75268 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References