Detailed information of TA system TA042900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4228892..4229517
• Replicon: chromosome
• Accession: NZ_AP026937
• Organism: Escherichia coli strain EC20-4B-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: OO845_RS20420
• Protein ID: WP_000911330.1
• Coordinates: 4229119..4229517 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: OO845_RS20415
• Protein ID: WP_000450524.1
• Coordinates: 4228892..4229119 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO845_RS20390 (4224695)	4224695..4225165	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
OO845_RS20395 (4225165)	4225165..4225737	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
OO845_RS20400 (4225883)	4225883..4226761	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
OO845_RS20405 (4226778)	4226778..4227812	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
OO845_RS20410 (4228025)	4228025..4228738	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
OO845_RS20415 (4228892)	4228892..4229119	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
OO845_RS20420 (4229119)	4229119..4229517	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
OO845_RS20425 (4229664)	4229664..4230527	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
OO845_RS20430 (4230542)	4230542..4232557	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
OO845_RS20435 (4232631)	4232631..4233329	+	699	WP_000679823.1	esterase	-
OO845_RS20440 (4233439)	4233439..4233639	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T42900 WP_000911330.1 NZ_AP026937:4229119-4229517 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T42900 NZ_AP026937:4229119-4229517 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT42900 WP_000450524.1 NZ_AP026937:4228892-4229119 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT42900 NZ_AP026937:4228892-4229119 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33