Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3197785..3198371 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag OO845_RS15345 Protein ID WP_042014162.1
Coordinates 3197785..3198015 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO845_RS15350 Protein ID WP_000593555.1
Coordinates 3198012..3198371 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS15335 (3193822) 3193822..3196557 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
OO845_RS15340 (3196557) 3196557..3197681 + 1125 WP_001314210.1 ABC transporter permease -
OO845_RS15345 (3197785) 3197785..3198015 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
OO845_RS15350 (3198012) 3198012..3198371 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO845_RS15355 (3198491) 3198491..3198892 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
OO845_RS15360 (3198898) 3198898..3199704 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
OO845_RS15365 (3199701) 3199701..3200465 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
OO845_RS15370 (3200465) 3200465..3201298 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
OO845_RS15375 (3201295) 3201295..3202239 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T42895 WP_042014162.1 NZ_AP026937:3197785-3198015 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T42895 NZ_AP026937:3197785-3198015 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42895 WP_000593555.1 NZ_AP026937:3198012-3198371 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42895 NZ_AP026937:3198012-3198371 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References