Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2417175..2417672 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO845_RS11795 Protein ID WP_001173343.1
Coordinates 2417175..2417348 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO845_RS11800 Protein ID WP_000398619.1
Coordinates 2417376..2417672 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS11785 (2413845) 2413845..2415302 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
OO845_RS11790 (2415539) 2415539..2417056 + 1518 WP_001295074.1 lysine--tRNA ligase -
OO845_RS11795 (2417175) 2417175..2417348 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO845_RS11800 (2417376) 2417376..2417672 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO845_RS11805 (2417899) 2417899..2418171 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO845_RS11810 (2418183) 2418183..2418413 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
OO845_RS11815 (2418594) 2418594..2420225 + 1632 WP_001216461.1 sensor histidine kinase -
OO845_RS11820 (2420222) 2420222..2420941 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42893 WP_001173343.1 NZ_AP026937:c2417348-2417175 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42893 NZ_AP026937:c2417348-2417175 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42893 WP_000398619.1 NZ_AP026937:c2417672-2417376 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42893 NZ_AP026937:c2417672-2417376 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References