Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2314668..2315263 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag OO845_RS11280 Protein ID WP_000239579.1
Coordinates 2314668..2315018 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag OO845_RS11285 Protein ID WP_001223208.1
Coordinates 2315012..2315263 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS11260 (2309944) 2309944..2310966 - 1023 WP_001339490.1 ABC transporter permease -
OO845_RS11265 (2310980) 2310980..2312482 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
OO845_RS11270 (2312792) 2312792..2313748 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
OO845_RS11275 (2314058) 2314058..2314588 + 531 WP_000055072.1 inorganic diphosphatase -
OO845_RS11280 (2314668) 2314668..2315018 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
OO845_RS11285 (2315012) 2315012..2315263 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
OO845_RS11290 (2315476) 2315476..2315817 - 342 WP_063100134.1 gamma-glutamylcyclotransferase -
OO845_RS11295 (2315820) 2315820..2319599 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T42892 WP_000239579.1 NZ_AP026937:c2315018-2314668 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T42892 NZ_AP026937:c2315018-2314668 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT42892 WP_001223208.1 NZ_AP026937:c2315263-2315012 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT42892 NZ_AP026937:c2315263-2315012 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References