Detailed information of TA system TA042890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1704241..1705078
• Replicon: chromosome
• Accession: NZ_AP026937
• Organism: Escherichia coli strain EC20-4B-2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: OO845_RS08425
• Protein ID: WP_000227784.1
• Coordinates: 1704536..1705078 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: OO845_RS08420
• Protein ID: WP_001297137.1
• Coordinates: 1704241..1704552 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO845_RS08395 (1699261)	1699261..1700208	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
OO845_RS08400 (1700230)	1700230..1702221	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
OO845_RS08405 (1702211)	1702211..1702825	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
OO845_RS08410 (1702825)	1702825..1703154	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
OO845_RS08415 (1703166)	1703166..1704056	+	891	WP_000971336.1	heme o synthase	-
OO845_RS08420 (1704241)	1704241..1704552	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
OO845_RS08425 (1704536)	1704536..1705078	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
OO845_RS08430 (1705134)	1705134..1706069	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
OO845_RS08435 (1706477)	1706477..1707841	+	1365	WP_001000978.1	MFS transporter	-
OO845_RS08440 (1707969)	1707969..1708460	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
OO845_RS08445 (1708628)	1708628..1709539	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T42890 WP_000227784.1 NZ_AP026937:1704536-1705078 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T42890 NZ_AP026937:1704536-1705078 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT42890 WP_001297137.1 NZ_AP026937:1704241-1704552 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT42890 NZ_AP026937:1704241-1704552 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3