Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1670164..1670782 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO845_RS08255 Protein ID WP_001291435.1
Coordinates 1670564..1670782 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO845_RS08250 Protein ID WP_000344800.1
Coordinates 1670164..1670538 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS08240 (1665253) 1665253..1666446 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OO845_RS08245 (1666469) 1666469..1669618 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
OO845_RS08250 (1670164) 1670164..1670538 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO845_RS08255 (1670564) 1670564..1670782 + 219 WP_001291435.1 HHA domain-containing protein Toxin
OO845_RS08260 (1670954) 1670954..1671505 + 552 WP_000102568.1 maltose O-acetyltransferase -
OO845_RS08265 (1671621) 1671621..1672091 + 471 WP_000136192.1 YlaC family protein -
OO845_RS08270 (1672255) 1672255..1673805 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO845_RS08275 (1673847) 1673847..1674200 - 354 WP_000878140.1 DUF1428 domain-containing protein -
OO845_RS08285 (1674579) 1674579..1674890 + 312 WP_000409911.1 MGMT family protein -
OO845_RS08290 (1674921) 1674921..1675493 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42889 WP_001291435.1 NZ_AP026937:1670564-1670782 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42889 NZ_AP026937:1670564-1670782 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42889 WP_000344800.1 NZ_AP026937:1670164-1670538 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42889 NZ_AP026937:1670164-1670538 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References