Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 655452..656090 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag OO845_RS03190 Protein ID WP_000813794.1
Coordinates 655914..656090 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag OO845_RS03185 Protein ID WP_001270286.1
Coordinates 655452..655868 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS03165 (650604) 650604..651545 - 942 WP_001251319.1 ABC transporter permease -
OO845_RS03170 (651546) 651546..652559 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
OO845_RS03175 (652577) 652577..653722 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
OO845_RS03180 (653967) 653967..655373 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
OO845_RS03185 (655452) 655452..655868 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
OO845_RS03190 (655914) 655914..656090 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
OO845_RS03195 (656312) 656312..656542 + 231 WP_000494244.1 YncJ family protein -
OO845_RS03200 (656634) 656634..658595 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
OO845_RS03205 (658668) 658668..659204 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
OO845_RS03210 (659296) 659296..660471 + 1176 WP_001236319.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 660511..661776 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T42888 WP_000813794.1 NZ_AP026937:c656090-655914 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T42888 NZ_AP026937:c656090-655914 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT42888 WP_001270286.1 NZ_AP026937:c655868-655452 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT42888 NZ_AP026937:c655868-655452 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References