Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5212064..5212689 Replicon chromosome
Accession NZ_AP026913
Organism Klebsiella pneumoniae strain Kp21r_f22

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag kp21f22_RS25585 Protein ID WP_004187928.1
Coordinates 5212064..5212447 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag kp21f22_RS25590 Protein ID WP_004150355.1
Coordinates 5212447..5212689 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
kp21f22_RS25570 (5209430) 5209430..5210332 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
kp21f22_RS25575 (5210329) 5210329..5210964 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
kp21f22_RS25580 (5210961) 5210961..5211890 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
kp21f22_RS25585 (5212064) 5212064..5212447 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
kp21f22_RS25590 (5212447) 5212447..5212689 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
kp21f22_RS25595 (5212883) 5212883..5213800 + 918 WP_004187929.1 alpha/beta hydrolase -
kp21f22_RS25600 (5213814) 5213814..5214755 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
kp21f22_RS25605 (5214800) 5214800..5215237 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
kp21f22_RS25610 (5215234) 5215234..5216094 - 861 WP_004146232.1 virulence factor BrkB family protein -
kp21f22_RS25615 (5216088) 5216088..5216687 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T42875 WP_004187928.1 NZ_AP026913:c5212447-5212064 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T42875 NZ_AP026913:c5212447-5212064 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT42875 WP_004150355.1 NZ_AP026913:c5212689-5212447 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT42875 NZ_AP026913:c5212689-5212447 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References