Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3960575..3961194 Replicon chromosome
Accession NZ_AP026913
Organism Klebsiella pneumoniae strain Kp21r_f22

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag kp21f22_RS19525 Protein ID WP_002892050.1
Coordinates 3960976..3961194 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag kp21f22_RS19520 Protein ID WP_002892066.1
Coordinates 3960575..3960949 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
kp21f22_RS19510 (3955727) 3955727..3956920 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
kp21f22_RS19515 (3956943) 3956943..3960089 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
kp21f22_RS19520 (3960575) 3960575..3960949 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
kp21f22_RS19525 (3960976) 3960976..3961194 + 219 WP_002892050.1 HHA domain-containing protein Toxin
kp21f22_RS19530 (3961353) 3961353..3961919 + 567 WP_002892030.1 maltose O-acetyltransferase -
kp21f22_RS19535 (3961891) 3961891..3962031 - 141 WP_004147370.1 hypothetical protein -
kp21f22_RS19540 (3962052) 3962052..3962522 + 471 WP_002892026.1 YlaC family protein -
kp21f22_RS19545 (3962497) 3962497..3963948 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
kp21f22_RS19550 (3964049) 3964049..3964747 + 699 WP_002892021.1 GNAT family protein -
kp21f22_RS19555 (3964744) 3964744..3964884 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
kp21f22_RS19560 (3964884) 3964884..3965147 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42870 WP_002892050.1 NZ_AP026913:3960976-3961194 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T42870 NZ_AP026913:3960976-3961194 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT42870 WP_002892066.1 NZ_AP026913:3960575-3960949 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT42870 NZ_AP026913:3960575-3960949 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References