Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5215980..5216605 Replicon chromosome
Accession NZ_AP026912
Organism Klebsiella pneumoniae strain Kp21_f22

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag OO849_RS25610 Protein ID WP_004187928.1
Coordinates 5215980..5216363 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OO849_RS25615 Protein ID WP_004150355.1
Coordinates 5216363..5216605 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO849_RS25595 (5213346) 5213346..5214248 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OO849_RS25600 (5214245) 5214245..5214880 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OO849_RS25605 (5214877) 5214877..5215806 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OO849_RS25610 (5215980) 5215980..5216363 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
OO849_RS25615 (5216363) 5216363..5216605 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OO849_RS25620 (5216799) 5216799..5217716 + 918 WP_004187929.1 alpha/beta hydrolase -
OO849_RS25625 (5217730) 5217730..5218671 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OO849_RS25630 (5218716) 5218716..5219153 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OO849_RS25635 (5219150) 5219150..5220010 - 861 WP_004146232.1 virulence factor BrkB family protein -
OO849_RS25640 (5220004) 5220004..5220603 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T42855 WP_004187928.1 NZ_AP026912:c5216363-5215980 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T42855 NZ_AP026912:c5216363-5215980 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT42855 WP_004150355.1 NZ_AP026912:c5216605-5216363 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT42855 NZ_AP026912:c5216605-5216363 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References