Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3964971..3965590 Replicon chromosome
Accession NZ_AP026912
Organism Klebsiella pneumoniae strain Kp21_f22

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag OO849_RS19545 Protein ID WP_002892050.1
Coordinates 3965372..3965590 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag OO849_RS19540 Protein ID WP_002892066.1
Coordinates 3964971..3965345 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO849_RS19530 (3960123) 3960123..3961316 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OO849_RS19535 (3961339) 3961339..3964485 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
OO849_RS19540 (3964971) 3964971..3965345 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
OO849_RS19545 (3965372) 3965372..3965590 + 219 WP_002892050.1 HHA domain-containing protein Toxin
OO849_RS19550 (3965749) 3965749..3966315 + 567 WP_002892030.1 maltose O-acetyltransferase -
OO849_RS19555 (3966287) 3966287..3966427 - 141 WP_004147370.1 hypothetical protein -
OO849_RS19560 (3966448) 3966448..3966918 + 471 WP_002892026.1 YlaC family protein -
OO849_RS19565 (3966893) 3966893..3968344 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
OO849_RS19570 (3968445) 3968445..3969143 + 699 WP_002892021.1 GNAT family protein -
OO849_RS19575 (3969140) 3969140..3969280 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
OO849_RS19580 (3969280) 3969280..3969543 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42850 WP_002892050.1 NZ_AP026912:3965372-3965590 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T42850 NZ_AP026912:3965372-3965590 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT42850 WP_002892066.1 NZ_AP026912:3964971-3965345 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT42850 NZ_AP026912:3964971-3965345 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References