Detailed information of TA system TA042825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3589312..3590149
• Replicon: chromosome
• Accession: NZ_AP026907
• Organism: Escherichia coli strain MY732

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: MY015_RS17430
• Protein ID: WP_000227784.1
• Coordinates: 3589607..3590149 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MY015_RS17425
• Protein ID: WP_001297137.1
• Coordinates: 3589312..3589623 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY015_RS17400 (3584332)	3584332..3585279	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
MY015_RS17405 (3585301)	3585301..3587292	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MY015_RS17410 (3587282)	3587282..3587896	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MY015_RS17415 (3587896)	3587896..3588225	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MY015_RS17420 (3588237)	3588237..3589127	+	891	WP_000971336.1	heme o synthase	-
MY015_RS17425 (3589312)	3589312..3589623	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MY015_RS17430 (3589607)	3589607..3590149	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
MY015_RS17435 (3590205)	3590205..3591140	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
MY015_RS17440 (3591548)	3591548..3592912	+	1365	WP_001000975.1	MFS transporter	-
MY015_RS17445 (3593040)	3593040..3593531	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
MY015_RS17450 (3593699)	3593699..3594610	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T42825 WP_000227784.1 NZ_AP026907:3589607-3590149 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T42825 NZ_AP026907:3589607-3590149 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT42825 WP_001297137.1 NZ_AP026907:3589312-3589623 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT42825 NZ_AP026907:3589312-3589623 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3