Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3555223..3555841 Replicon chromosome
Accession NZ_AP026907
Organism Escherichia coli strain MY732

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MY015_RS17260 Protein ID WP_001291435.1
Coordinates 3555623..3555841 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MY015_RS17255 Protein ID WP_000344800.1
Coordinates 3555223..3555597 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY015_RS17245 (3550312) 3550312..3551505 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MY015_RS17250 (3551528) 3551528..3554677 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MY015_RS17255 (3555223) 3555223..3555597 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MY015_RS17260 (3555623) 3555623..3555841 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MY015_RS17265 (3556012) 3556012..3556563 + 552 WP_000102578.1 maltose O-acetyltransferase -
MY015_RS17270 (3556679) 3556679..3557149 + 471 WP_000136192.1 YlaC family protein -
MY015_RS17275 (3557313) 3557313..3558863 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MY015_RS17280 (3558905) 3558905..3559258 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MY015_RS17290 (3559637) 3559637..3559948 + 312 WP_000409911.1 MGMT family protein -
MY015_RS17295 (3559979) 3559979..3560551 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42824 WP_001291435.1 NZ_AP026907:3555623-3555841 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42824 NZ_AP026907:3555623-3555841 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42824 WP_000344800.1 NZ_AP026907:3555223-3555597 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42824 NZ_AP026907:3555223-3555597 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References